Anti-ME2 Antibody Picoband®

SKU: A01380-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Monkey,Mouse,Rat

Applications: ELISA,Flow Cytometry,IF,IHC,ICC,WB

Application Details: Western blot, 0.1-0.25μg/ml, Human, Mouse, Monkey, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
ELISA, 0.1-0.5μg/ml, -

Gene Name: ME2

Specificity: No cross reactivity with other proteins.

Background: NAD-dependent malic enzyme, mitochondrial is a protein that in humans is encoded by the ME2 gene. It is mapped to 18q21.2. This gene encodes a mitochondrial NAD-dependent malic enzyme, a homotetrameric protein, that catalyzes the oxidative decarboxylation of malate to pyruvate. It had previously been weakly linked to a syndrome known as Friedreich ataxia that has since been shown to be the result of mutation in a completely different gene. Certain single-nucleotide polymorphism haplotypes of this gene have been shown to increase the risk for idiopathic generalized epilepsy. Alternatively spliced transcript variants encoding different isoforms found for this gene.

Immunogen: E.coli-derived human ME2 recombinant protein (Position: K26-E584).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Burchell, A., Crosby, A., Cohen, P. T. W. Human mitochondrial malic enzyme variants: properties of the different polymorphic forms. Ann. Hum. Genet. 41: 1-7, 1977. 2. Champion, M. J., Brown, J. A., Shows, T. B. Assignment of cytoplasmic alpha-mannosidase (MAN-A) and confirmation of mitochondrial isocitrate dehydrogenase (IDH-M) to the q11-qter region of chromosome 15 in man. Cytogenet. Cell Genet. 22: 498-502, 1978. 3. Cohen, P. T. W., Omenn, G. S. Genetic variation of the cytoplasmic and mitochondrial malic enzymes in the monkey: Macaca nemestrina. Biochem. Genet. 7: 289-301, 1972.

Uniprot ID: P23368

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 65 kDa

Calculated Molecular Weight: 90056 MW

Gene ID: 4200

Protein Name: NAD-dependent malic enzyme, mitochondrial

Gene Full Name: malic enzyme 2

Synonyms: Malic enzyme 2; ME2; NAD ME