Anti-MDMX/MDM4 Antibody Picoband®

SKU: A01889-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: MDM4

Specificity:

Background: Protein Mdm4?is a?protein?that in humans is encoded by the?MDM4?gene. This gene encodes a nuclear protein that contains a p53 binding domain at the N-terminus and a RING finger domain at the C-terminus, and shows structural similarity to p53-binding protein MDM2. Both proteins bind the p53 tumor suppressor protein and inhibit its activity, and have been shown to be overexpressed in a variety of human cancers. However, unlike MDM2 which degrades p53, this protein inhibits p53 by binding its transcriptional activation domain. This protein also interacts with MDM2 protein via the RING finger domain, and inhibits the latter's degradation. So this protein can reverse MDM2-targeted degradation of p53, while maintaining suppression of p53 transactivation and apoptotic functions. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

Immunogen: E.coli-derived human MDMX/MDM4 recombinant protein (Position: A8-A490).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Entrez Gene: MDM4 Mdm4, transformed 3T3 cell double minute 4, p53 binding protein (mouse). 2. Kadakia M, Brown TL, McGorry MM, Berberich SJ (Dec 2002). MdmX inhibits Smad transactivation. Oncogene21 (57): 8776–85. 3. Shvarts A, Bazuine M, Dekker P, Ramos YF, Steegenga WT, Merckx G, van Ham RC, van der Houven van Oordt W, van der Eb AJ, Jochemsen AG (Sep 1997). Isolation and identification of the human homolog of a new p53-binding protein, Mdmx. Genomics 43 (1): 34–42.

Uniprot ID: O15151

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 55 kDa

Calculated Molecular Weight: 146424 MW

Gene ID: 4194

Protein Name: Protein Mdm4

Gene Full Name: MDM4 regulator of p53

Synonyms: Double minute 4 protein; HDMX; Mdm2 like p53 binding protein; MDM4; MDMX; MRP1; p53 binding protein Mdm4; Protein Mdm4; Protein Mdmx