Anti-MCM6 Antibody Picoband® (monoclonal, 3F13C4)

SKU: M02755-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Gene Name: MCM6

Specificity:

Background: MCM6(Minichromosome maintenance, s. pombe, homolog of, 6) is a protein that in humans is encoded by the MCM6 gene. MCM6 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The MCM genes were originally identified in yeast defective in minichromosome maintenance and have since been shown to play roles in the progression of the cell cycle; many are cell division control genes. The MCM6 gene is mapped on 2q21.3. Mcm 6 has recently been shown to interact strongly Cdt1 at defined residues, by mutating these target residues Wei et al. observed lack of Cdt1 recruitment of Mcm2-7 to the pre-RC. An approximately 200-kb region surrounding the C/T(-13910) polymorphism in MCM6 intron 13 functioned as an enhancer of the lactase gene promoter in intestinal cell culture.

Immunogen: E.coli-derived human MCM6 recombinant protein (Position: Q14-D821).

Clonality: Monoclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Bersaglieri, T., Sabeti, P. C., Patterson, N., Vanderploeg, T., Schaffner, S. F., Drake, J. A., Rhodes, M., Reich, D. E., Hirschhorn, J. N. Genetic signatures of strong recent positive selection at the lactase gene. Am. J. Hum. Genet. 74: 1111-1120, 2004. 2. Coelho, M., Luiselli, D., Bertorelle, G., Lopes, A. I., Seixas, S., Destro-Bisol, G., Rocha, J. Microsatellite variation and evolution of human lactase persistence. Hum. Genet. 117: 329-339, 2005. 3. Enattah, N. S., Sahi, T., Savilahti, E., Terwilliger, J. D., Peltonen, L., Jarvela, I. Identification of a variant associated with adult-type hypolactasia. Nature Genet. 30: 233-237, 2002.

Uniprot ID: Q14566

Host: Mouse

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Mouse IgG1

Phospho_site:

Clone Number: Clone: 3F13C4

Observed Molecular Weight: 105 kDa

Calculated Molecular Weight:

Gene ID: 4175

Protein Name: DNA replication licensing factor MCM6

Gene Full Name: minichromosome maintenance complex component 6

Synonyms: MCG40308; MCM6; Mis5; P105MCM