Anti-Matrix metalloproteinase-14 MMP14 Antibody Picoband®

SKU: PA2147

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: IHC,WB

Application Details: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Gene Name: MMP14

Specificity: No cross reactivity with other proteins.

Background: Matrix metalloproteinase-14, also called MT-MMP 1, is an enzyme that in humans is encoded by the MMP14 gene. The protein encoded by this gene is a member of the membrane-type MMP subfamily. This gene is mapped to 14q11.2. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. This gene may be involved in actin cytoskeleton reorganization by cleaving PTK7. It acts as a positive regulator of cell growth and migration via activation of MMP15.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human MMP14, different from the related mouse and rat sequences by one amino acid.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Chun, T.-H., Hotary, K. B., Sabeh, F., Saltiel, A. R., Allen, E. D., Weiss, S. J. A pericellular collagenase s the 3-dimensional development of white adipose tissue. Cell 125: 577-591, 2006. 2. Oh, J., Takahashi, R., Adachi, E., Kondo, S., Kuratomi, S., Noma, A., Alexander, D. B., Motoda, H., Okada, A., Seiki, M., Itoh, T., Itohara, S., Takahashi, C., Noda, M. Mutations in two matrix metalloproteinase genes, MMP-2, and MT1-MMP, are synthetic lethal in mice. Oncogene 23: 5041-5048, 2004. 3. Ueda, M., Yamashita, Y., Takehara, M., Terai, Y., Kumagai, K., Ueki, K., Kanda, K., Yamaguchi, H., Akise, D., Hung, Y.-C., Ueki, M. Survivin gene expression in endometriosis. J. Clin. Endocr. Metab. 87: 3452-3459, 2002.

Uniprot ID: P50281

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 150 kDa

Calculated Molecular Weight: 65894 MW

Gene ID: 4323

Protein Name: Matrix metalloproteinase-14

Gene Full Name: matrix metallopeptidase 14

Synonyms: Matrix metalloproteinase 14; MMP 14; MMP X1; MMP14; MT MMP 1; MT1 MMP; MT1MMP; MT1-MMP; MTMMP1