Anti-MATK Antibody Picoband®

SKU: A02050-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: MATK

Specificity:

Background: Megakaryocyte-associated tyrosine-protein kinase is an enzyme that in humans is encoded by the MATK gene. The protein encoded by this gene has amino acid sequence similarity to Csk tyrosine kinase and has the structural features of the CSK subfamily: SRC homology SH2 and SH3 domains, a catalytic domain, a unique N terminus, lack of myristylation signals, lack of a negative regulatory phosphorylation site, and lack of an autophosphorylation site. This protein is thought to play a significant role in the signal transduction of hematopoietic cells. It is able to phosphorylate and inactivate Src family kinases, and may play an inhibitory role in the control of T-cell proliferation. This protein might be involved in signaling in some cases of breast cancer. Three alternatively spliced transcript variants that encode different isoforms have been described for this gene.

Immunogen: E.coli-derived human MATK recombinant protein (Position: S18-Q298). Human MATK shares 88.5% and 91.4% amino acid (aa) sequence identity with mouse and rat MATK, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Avraham, S., Jiang, S., Ota, S., Fu, Y., Deng, B., Dowler, L. L., White, R. A., Avraham, H. Structural and functional studies of the intracellular tyrosine kinase MATK gene and its translated product. J. Biol. Chem. 270: 1833-1842, 1995. 2. Bennett, B. D., Cowley, S., Jiang, S., London, R., Deng, B., Grabarek, J., Groopman, J. E., Goeddel, D. V., Avraham, H. Identification and characterization of a novel tyrosine kinase from megakaryocytes. J. Biol. Chem. 269: 1068-1074, 1994. 3. Klages, S., Adam, D., Class, K., Fargnoli, J., Bolen, J. B., Penhallow, R. C. Ctk: a protein-tyrosine kinase related to Csk that defines an enzyme family. Proc. Nat. Acad. Sci. 91: 2597-2601, 1994.

Uniprot ID: P42679

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity:

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 52 kDa

Calculated Molecular Weight: 56 kDa

Gene ID: 4145

Protein Name: Megakaryocyte-associated tyrosine-protein kinase

Gene Full Name: megakaryocyte-associated tyrosine kinase

Synonyms: MATK; CTK; HYL; Megakaryocyte-associated tyrosine-protein kinase; CSK homologous kinase; CHK; Hematopoietic consensus tyrosine-lacking kinase; Protein kinase HYL; Tyrosine-protein kinase CTK