Anti-MARCKS Antibody Picoband®

SKU: A04133-4

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,Flow Cytometry

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Gene Name: MARCKS

Specificity:

Background: The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human MARCKS.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Blackshear, P. J. The MARCKS family of cellular protein kinase C substrates. J. Biol. Chem. 268: 1501-1504, 1993. 2. Blackshear, P. J., Tuttle, J. S., Oakey, R. J., Seldin, M. F., Chery, M., Philippe, C., Stumpo, D. J. Chromosomal mapping of the human (MACS) and mouse (Macs) genes encoding the MARCKS protein. Genomics 14: 168-174, 1992. 3. Boyle, J. M., Hey, Y., Myers, K., Stern, P. L., Grzeschik, F.-H., Ikehara, Y., Misumi, Y., Fox, M. Regional localization of a trophoblast antigen-related sequence and 16 other sequences to human chromosome 6q using somatic cell hybrids. Genomics 12: 693-698, 1992.

Uniprot ID: P29966

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 80 kDa

Calculated Molecular Weight: 32 kDa

Gene ID: 4082

Protein Name: Myristoylated alanine-rich C-kinase substrate

Gene Full Name: myristoylated alanine rich protein kinase C substrate

Synonyms: Macs, Marcks, PKCSL