Anti-Lck Antibody Picoband®

SKU: PB9808

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: Flow Cytometry,IF,IHC,IHC-F,ICC,WB

Application Details: Western blot, 0.1-0.5μg/ml, Human, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Mouse
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human

Gene Name: LCK

Specificity: No cross reactivity with other proteins.

Background: Lck (or lymphocyte-specific protein tyrosine kinase) is a 56 kDa protein that is found inside specializedcells of the immune system called lymphocytes. The human LCK gene is mapped to chromosome 1p35-p32. This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants, encoding the same protein, have been described.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Lck, different from the related mouse and rat sequences by three amino acids.

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Anderson, S. J., Levin, S. D., Perlmutter, R. M. Protein tyrosine kinase p56(lck) controls allelic exclusion of T-cell receptor beta-chain genes. Nature 365: 552-554, 1993. 2. Burnett, R. C., David, J.-C., Harden, A. M., Le Beau, M. M., Rowley, J. D., Diaz, M. O. The LCK gene is involved in the t(1;7)(p34;q34) in the T-cell acute lymphoblastic leukemia derived cell line, HSB-2. Genes Chromosomes Cancer 3: 461-467, 1991. 3. Marth, J. D., Disteche, C., Pravtcheva, D., Ruddle, F., Krebs, E. G., Perlmutter, R. M. Localization of a lymphocyte-specific protein tyrosine kinase gene (lck) at a site of frequent chromosomal abnormalities in human lymphomas. Proc. Nat. Acad. Sci. 83: 7400-7404, 1986.

Uniprot ID: P06239

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 58 kDa

Calculated Molecular Weight: 58001 MW

Gene ID: 3932

Protein Name: Tyrosine-protein kinase Lck

Gene Full Name: LCK proto-oncogene, Src family tyrosine kinase

Synonyms: LCK; LSK; p56 LCK; p56lck; pp58lck; Protein YT16; Proto oncogene Lck; Tyrosine protein kinase Lck; YT16