Anti-JUNB Antibody Picoband®

SKU: A01825-3

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Gene Name: JUNB

Specificity:

Background: Transcription factor jun-B (JUNB) is a protein that in humans is encoded by the JUNB gene. It is mapped to 19p13.2. JUNB is a transcription factor involved in regulating gene activity following the primary growth factor response. It binds to the DNA sequence 5'-TGA[CG]TCA-3', and a large fraction (over 50%) of the JUNB locus is contained in these flanking evolutionarily conserved sequences (FECS), which may be required for effecting the proper transcriptional regulation of this gene. What's more, the expression of JUNB gene might be involved in terminal granulocyte differentiation or in regulating granulocyte functionality.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human JUNB, which shares 88.9% amino acid (aa) sequence identity with both mouse and rat JUNB.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Schütte J, Viallet J, Nau M, Segal S, Fedorko J, Minna J (Feb 1990). jun-B inhibits and c-fos stimulates the transforming and trans-activating activities of c-jun. Cell 59 (6): 987–997. 2. Mollinedo F, Vaquerizo MJ, Naranjo JR (1991). Expression of c-jun, jun B and jun D proto-oncogenes in human peripheral-blood granulocytes. Biochem. J. 273(Pt 2): 477–9. 3. Phinney DG, Tseng SW, Ryder K (1996). Complex genetic organization of junB: multiple blocks of flanking evolutionarily conserved sequence at the murine and human junB loci. Genomics 28 (2): 228–234.

Uniprot ID: P17275

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 42-45 kDa

Calculated Molecular Weight: 18080 MW

Gene ID: 3726

Protein Name: Transcription factor JunB

Gene Full Name: JunB proto-oncogene, AP-1 transcription factor subunit

Synonyms: AP 1; jun B proto oncogene; JUNB; Transcription factor jun B