Anti-IRAK-1/IRAK1 Antibody Picoband®

SKU: A01021-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: Flow Cytometry,IF,IHC,ICC,WB

Application Details: Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human

Gene Name: IRAK1

Specificity: No cross reactivity with other proteins.

Background: Interleukin-1 receptor-associated kinase 1, also called IRAK1, is an enzyme that in humans is encoded by the IRAK1 gene. By radiation hybrid analysis, this gene is mapped to chromosome Xq28. Serine/threonine-protein kinase plays a critical role in initiating innate immune response against foreign pathogens. This gene involved in Toll-like receptor (TLR) and IL-1R signaling pathways. This gene encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This gene is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human IRAK-1/IRAK1, identical to the related mouse and rat sequences.

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Chassin, C., Kocur, M., Pott, J., Duerr, C. U., Gutle, D., Lotz, M., Hornef, M. W. miR-146a mediates protective innate immune tolerance in the neonate intestine. Cell Host Microbe 8: 358-368, 2010. 2. Jacob, C. O., Zhu, J., Armstrong, D. L., Yan, M., Han, J., Zhou, X. J., Thomas, J. A., Reiff, A., Myones, B. L., Ojwang, J. O., Kaufman, K. M., Klein-Gitelman, M., and 17 others. Identification of IRAK1 as a risk gene with critical role in the pathogenesis of systemic lupus erythematosus. Proc. Nat. Acad. Sci. 106: 6256-6261, 2009.

Uniprot ID: P51617

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 77 kDa

Calculated Molecular Weight: 47579 MW

Gene ID: 3654

Protein Name: Interleukin-1 receptor-associated kinase 1

Gene Full Name: interleukin 1 receptor associated kinase 1

Synonyms: IRAK; IRAK 1; IRAK1; pelle