Anti-Involucrin/IVL Antibody Picoband®

SKU: PB9712

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: WB

Application Details: Western blot, 0.1-0.5μg/ml, Human

Gene Name: IVL

Specificity: No cross reactivity with other proteins.

Background: Involucrin?is a?protein?component of human skin and in humans is encoded by the?IVL?gene. It is a highly reactive, soluble,?transglutaminase?substrate?protein?present in?keratinocytes?of?epidermisand other?stratified squamous epithelia. It first appears in the?cell?cytosol, but ultimately becomes cross-linked to?membrane proteins?by transglutaminase thus helping in the formation of an insoluble envelope beneath theplasma membrane?functioning as a?glutamyl?donor during assembly of the cornified envelope. Additionally, Involucrin is synthesised in the?stratum spinosum?and cross linked in the?stratum granulosum?by thetransglutaminase?enzyme that makes it highly stable. Thus it provides structural support to the cell, thereby allowing the cell to resist invasion by micro-organisms.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Involucrin.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Djian P, Phillips M, Easley K, Huang E, Simon M, Rice RH, Green H (November 1993). The involucrin genes of the mouse and the rat: study of their shared repeats. Mol. Biol. Evol. 10 (6): 1136–49. 2. Eckert RL, Green H (Sep 1986). Structure and evolution of the human involucrin gene. Cell 46 (4): 583–9. 3. Green H, Djian P (November 1992). Consecutive actions of different gene-altering mechanisms in the evolution of involucrin. Mol. Biol. Evol. 9 (6): 977–1017.

Uniprot ID: P07476

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 120 kDa

Calculated Molecular Weight: 68479 MW

Gene ID: 3713

Protein Name: Involucrin

Gene Full Name: involucrin

Synonyms: involucrin; IVL