Anti-INSIG1 Antibody Picoband®

SKU: A02026-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: INSIG1

Specificity:

Background: Insulin induced gene 1, also known as INSIG1, is a protein which in humans is encoded by the INSIG1 gene. This gene encodes an endoplasmic reticulum membrane protein that regulates cholesterol metabolism, lipogenesis, and glucose homeostasis. The encoded protein has six transmembrane helices which contain an effector protein binding site. It binds the sterol-sensing domains of sterol regulatory element-binding protein (SREBP) cleavage-activating protein (SCAP) and 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA reductase), and is essential for the sterol-mediated trafficking of these two proteins. It promotes the endoplasmic reticulum retention of SCAP and the ubiquitin-mediated degradation of HMG-CoA reductase. Alternative splicing results in multiple transcript variants.

Immunogen: E.coli-derived human INSIG1 recombinant protein (Position: M1-R87).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Diamond, R. H., Du, K., Lee, V. M., Mohn, K. L., Haber, B. A., Tewari, D. S., Taub, R. Novel delayed-early and highly insulin-induced growth response genes: identification of HRS, a potential regulator of alternative pre-mRNA splicing. J. Biol. Chem. 268: 15185-15192, 1993. 2. Engelking, L. J., Liang, G., Hammer, R. E., Takaishi, K., Kuriyama, H., Evers, B. M., Li, W.-P., Horton, J. D., Goldstein, J. L., Brown, M. S. Schoenheimer effect explained--feedback regulation of cholesterol synthesis in mice mediated by Insig proteins. J. Clin. Invest. 115: 2489-2498, 2005. 3. Gong, Y., Lee, J. N., Brown, M. S., Goldstein, J. L., Ye, J. Juxtamembranous aspartic acid in Insig-1 and Insig-2 is required for cholesterol homeostasis. Proc. Nat. Acad. Sci. 103: 6154-6159, 2006.

Uniprot ID: O15503

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 66 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 3638

Protein Name: Insulin-induced gene 1 protein

Gene Full Name: insulin induced gene 1

Synonyms: INSIG1; Insulin-induced gene 1 protein; INSIG-1