Anti-IKKi/IKKe/IKBKE Antibody Picoband®

SKU: A01816-2

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: WB,ELISA

Application Details: Western blot, 0.25-0.5μg/ml, Human
ELISA, 0.1-0.5μg/ml, -

Gene Name: IKBKE

Specificity:

Background: Inhibitor of nuclear factor kappa-B kinase subunit epsilon also known as I-kappa-B kinase epsilon or IKK-epsilon is an enzyme that in humans is encoded by the IKBKE gene. IKBKE is a noncanonical I-kappa-B kinase (IKK) that is essential for regulating antiviral signaling pathways. IKBKE has also been identified as a breast cancer oncogene and is amplified and overexpressed in over 30% of breast carcinomas and breast cancer cell line.

Immunogen: E.coli-derived human IKKi/IKKe/IKBKE recombinant protein (Position: Q318-V716).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Chiang, S.-H., Bazuine, M., Lumeng, C. N., Geletka, L. M., Mowers, J., White, N. M., Ma, J.-T., Zhou, J., Qi, N., Westcott, D., Delproposto, J. B., Blackwell, T. S., Yull, F. E., Saltiel, A. R. The protein kinase IKK-epsilon regulates energy balance in obese mice. Cell 138: 961-975, 2009. 2. Hutti, J. E., Shen, R. R., Abbott, D. W., Zhou, A. Y., Sprott, K. M., Asara, J. M., Hahn, W. C., Cantley, L. C. Phosphorylation of the tumor suppressor CYLD by the breast cancer oncogene IKK-epsilon promotes cell transformation. Molec. Cell 34: 461-472, 2009. 3. Nagase, T., Seki, N., Tanaka, A., Ishikawa, K., Nomura, N. Prediction of the coding sequences of unidentified human genes. IV. The coding sequences of 40 new genes (KIAA0121-KIAA0160) deduced by analysis of cDNA clones from human cell line KG-1. DNA Res. 2: 167-174, 1995.

Uniprot ID: Q14164

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 80 kDa

Calculated Molecular Weight:

Gene ID: 9641

Protein Name: Inhibitor of nuclear factor kappa-B kinase subunit epsilon

Gene Full Name: inhibitor of nuclear factor kappa B kinase subunit epsilon

Synonyms: inhibitor of nuclear factor kappa B kinase subunit epsilon; IKK-E; IKK-i; IKKE; IKKI; IKBKE; IKKi; IKKe