Anti-HLA-DPB1 Antibody Picoband®

SKU: A00487

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: IHC,WB

Application Details: Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human

Gene Name: HLA-DPB1

Specificity: No cross reactivity with other proteins.

Background: HLA class II histocompatibility antigen, DP (W2) beta chain is a protein that in humans is encoded by the HLA-DPB1 gene. HLA-DPB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta chain (DPB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DP molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules.

Immunogen: E. coli-derived human HLA-DPB1 recombinant protein (Position: R30-A258).

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Entrez Gene: HLA-DPB1 major histocompatibility complex, class II, DP beta 1. 2. Korioth F, Hartung K, Deicher H, Frey J (1992). A new HLA-DPB1 allele from a patient with systemic lupus erythematosus. Tissue Antigens. 39 (4): 216–9. 3. Mitsunaga S, Kuwata S, Tokunaga K, et al. (1992). Family study on HLA-DPB1 polymorphism: linkage analysis with HLA-DR/DQ and two new alleles. Hum. Immunol. 34 (3): 203–11.

Uniprot ID: P04440

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 29 kDa

Calculated Molecular Weight: 29159 MW

Gene ID: 3115

Protein Name: HLA class II histocompatibility antigen, DP beta 1 chain

Gene Full Name: major histocompatibility complex, class II, DP beta 1

Synonyms: DPB1; HLA DP1B; HLA DPB1; HLA-DPB1; MHC class II antigen DPB1