Anti-HEBP1 Antibody Picoband®

SKU: A00271-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: HEBP1

Specificity:

Background: Heme binding protein 1 is a protein that in humans is encoded by the HEBP1 gene. The full-length protein encoded by this gene is an intracellular tetrapyrrole-binding protein. This protein includes a natural chemoattractant peptide of 21 amino acids at the N-terminus, which is a natural ligand for formyl peptide receptor-like receptor 2 (FPRL2) and promotes calcium mobilization and chemotaxis in monocytes and dendritic cells.

Immunogen: E.coli-derived human HEBP1 recombinant protein (Position: K40-R162). Human HEBP1 shares 87.1% amino acid (aa) sequence identity with mouse HEBP1.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Blackmon, B. J., Dailey, T. A., Lianchun, X., Dailey, H. A. Characterization of a human and mouse tetrapyrrole-binding protein. Arch. Biochem. Biophys. 407: 196-201, 2002. 2. Devosse, T., Dutoit, R., Migeotte, I., De Nadai, P., Imbault, V., Communi, D., Salmon, I., Parmentier, M. Processing of HEBP1 by cathepsin D gives rise to F2L, the agonist of formyl peptide receptor 3. J. Immun. 187: 1475-1485, 2011. 3. Dias, J. S., Macedo, A. L., Ferreira, G. C., Peterson, F. C., Volkman, B. F., Goodfellow, B. J. The first structure from the SOUL/HBP family of heme-binding proteins, murine P22HBP. J. Biol. Chem. 281: 31553-31561, 2006. Note: Erratum: J. Biol. Chem. 281: 38966 only, 2006.

Uniprot ID: Q9NRV9

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 21 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 50865

Protein Name: Heme-binding protein 1

Gene Full Name: heme binding protein 1

Synonyms: HBP; HEBP; HEBP1; heme binding protein 1; p22HBP