Anti-GSDMD Antibody Picoband® (monoclonal, 6D11)

SKU: M02842

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,IHC,ICC,IF,Flow Cytometry

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Gene Name: GSDMD

Specificity:

Background: Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described.

Immunogen: E.coli-derived human GSDMD recombinant protein (Position: M1-H484).

Clonality: Monoclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Ruhl, S., Shkarina, K., Demarco, B., Heilig, R., Santos, J. C., Broz, P. ESCRT-dependent membrane repair negatively regulates pyroptosis downstream of GSDMD activation. Science 362: 956-960, 2018. 2. Shi, J., Zhao, Y., Wang, K., Shi, X., Wang, Y., Huang, H., Zhuang, Y., Cai, T., Wang, F., Shao, F. Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death. Nature 526: 660-665, 2015. 3. Zhu, Q., Zheng, M., Balakrishnan, A., Karki, R., Kanneganti, T.-D. Gasdermin D promotes AIM2 inflammasome activation and is required for host protection against Francisella novicida. J. Immun. 201: 3662-3668, 2018.

Uniprot ID: P57764

Host: Mouse

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Mouse IgG2b

Phospho_site:

Clone Number: Clone: 6D11

Observed Molecular Weight: 53 kDa

Calculated Molecular Weight:

Gene ID: 79792

Protein Name: Gasdermin-D

Gene Full Name: gasdermin D

Synonyms: DF5L; DFNA5L; FKSG10; gasdermin D; GSDMD; GSDMDC1; GSDMD-NT