Anti-GRP94/HSP90B1 Antibody Picoband®

SKU: PA1340

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Rat

Applications: IHC,IHC-F,ICC,WB

Application Details: Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat
Immunocytochemistry , 0.5-1μg/ml, Human

Gene Name: HSP90B1

Specificity: No cross reactivity with other proteins.

Background: Heat shock protein 90kDa beta member 1  (HSP90B1), known also as endoplasmin, GRP94, is a chaperone protein that in humans is encoded by the HSP90B1 gene. It is mapped to chromosome 12q23.3. This gene encodes a member of a family of adenosine triphosphate (ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human GRP94, identical to the related mouse and rat sequences.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Rebbe NF, Hickman WS, Ley TJ, Stafford DW, Hickman S (Oct 1989). Nucleotide sequence and regulation of a human 90-kDa heat shock protein gene. J Biol Chem 264 (25): 15006–11. 2. Chen B, Piel WH, Gui L, Bruford E, Monteiro A (Dec 2005). The HSP90 family of genes in the human genome: insights into their divergence and evolution. Genomics 86 (6): 627–37. 3. Entrez Gene: HSP90AB1 Heat shock protein 90kDa alpha (cytosolic), class B member 4. Chen, B., Piel, W. H., Gui, L., Bruford, E., Monteiro, A. The HSP90 family of genes in the human genome: insights into their divergence and evolution. Genomics 86: 627-637, 2005. 5. Prodromou C, Panaretou B, Chohan S, Siligardi G, O'Brien R, Ladbury JE, Roe SM, Piper PW, Pearl LH (August 2000). The ATPase cycle of Hsp90 drives a molecular 'clamp' via transient dimerization of the N-terminal domains. EMBO J. 19 (16): 4383–92.

Uniprot ID: P14625

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 100 kDa

Calculated Molecular Weight: 92469 MW

Gene ID: 7184

Protein Name: Endoplasmin

Gene Full Name: heat shock protein 90 beta family member 1

Synonyms: Cell and organelle markers; ECGP; endoplasmic reticulum marker; Endoplasmin; GP96; gp96 homolog; GRP 94; GRP94; HSP90B1; TRA1; Tumor rejection antigen 1