Anti-Grancalcin/GCA Antibody Picoband®

SKU: A01237

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,IF,IHC,ICC,WB,Flow Cytometry

Application Details: Western blot, 0.1-0.5μg/ml
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml
Immunocytochemistry/Immunofluorescence, 5 μg/ml
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells
ELISA, 0.1-0.5μg/ml

Gene Name: GCA

Specificity: No cross reactivity with other proteins.

Background: Grancalcin is a protein that in humans is encoded by the GCA gene. This gene is mapped to 2q24.2. This gene product, grancalcin, is a calcium-binding protein abundant in neutrophils and macrophages. It belongs to the penta-EF-hand subfamily of proteins which includes sorcin, calpain, and ALG-2. Grancalcin localization is dependent upon calcium and magnesium. In the absence of divalent cation, grancalcin localizes to the cytosolic fraction; with magnesium alone, it partitions with the granule fraction; and in the presence of magnesium and calcium, it associates with both the granule and membrane fractions, suggesting a role for grancalcin in granule-membrane fusion and degranulation.

Immunogen: E. coli-derived human Grancalcin/GCA recombinant protein (Position: A42-I217).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Boyhan A, Casimir CM, French JK, Teahan CG, Segal AW (Mar 1992). Molecular cloning and characterization of grancalcin, a novel EF-hand calcium-binding protein abundant in neutrophils and monocytes. J Biol Chem. 267 (5): 2928–33. 2. Teahan CG, Totty NF, Segal AW (Oct 1992). Isolation and characterization of grancalcin, a novel 28 kDa EF-hand calcium-binding protein from human neutrophils. Biochem J. 286. ( Pt 2): 549–54. 3. Hansen C, Tarabykina S, la Cour JM, Lollike K, Berchtold MW (Jun 2003). The PEF family proteins sorcin and grancalcin interact in vivo and in vitro. FEBS Lett. 545 (2-3): 151–4. 4. Entrez Gene: GCA grancalcin, EF-hand calcium binding protein.

Uniprot ID: P28676

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 25 kDa

Calculated Molecular Weight: 123799 MW

Gene ID: 25801

Protein Name: Grancalcin

Gene Full Name: grancalcin

Synonyms: GCA; GCL; Grancalcin