Anti-GLT25D1/COLGALT1 Antibody Picoband®

SKU: A12583-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: COLGALT1

Specificity:

Background: The protein encoded by this gene is one of two enzymes that transfers galactose moieties to hydroxylysine residues of collagen and mannose binding lectin. This gene is constitutively expressed and encodes a soluble protein that localizes to the endoplasmic reticulum.

Immunogen: E.coli-derived human COLGALT1 recombinant protein (Position: A34-D454). Human GLT25D1/COLGALT1 shares 93.6% amino acid (aa) sequence identity with mouse COLGALT1.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Baumann, S., Hennet, T. Collagen accumulation in osteosarcoma cells lacking GLT25D1 collagen galactosyltransferase. J. Biol. Chem. 291: 18514-18524, 2016. 2. Hartz, P. A. Personal Communication. Baltimore, Md. June 9, 2017. 3. Liefhebber, J. M. P., Punt, S., Spaan, W. J. M., van Leeuwen, H. C. The human collagen beta(1-O)galactosyltransferase, GLT25D1, is a soluble endoplasmic reticulum localized protein. BMC Cell Biol. 11: 33, 2010. Note: Electronic Article.

Uniprot ID: Q8NBJ5

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity:

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 85 kDa

Calculated Molecular Weight:

Gene ID: 79709

Protein Name: Procollagen galactosyltransferase 1

Gene Full Name: collagen beta(1-O)galactosyltransferase 1

Synonyms: GLT25D1