Anti-GATA3 Antibody Picoband®

SKU: A00593-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: Flow Cytometry,IF,IHC,ICC,WB

Application Details: Western blot, 0.25-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Immunocytochemistry/Immunofluorescence, 4μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human

Gene Name: GATA3

Specificity:

Background: Trans-acting T-cell-specific transcription factor GATA-3, also named HDR or GATA3, is a protein that in humans is encoded by the GATA3 gene. It is mapped to 10p14. This gene belongs to the GATA family of transcription factors. It regulates luminal epithelial cell differentiation in the mammary gland. The protein contains two GATA-type zinc fingers, it is an important regulator of T cell development and plays an important role in endothelial cell biology. GATA3 has been shown to promote the secretion of IL-4, IL-5, and IL-13 from Th2 cells, and induces the differentiation of Th0 cells towards this T cell subtype while suppressing their differentiation towards Th1 cells.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human GATA3, which shares 86.4% and 90.9% amino acid (aa) sequence identity with mouse and rat GATA3, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Joulin V, Bories D, Eleouet JF, Labastie MC, Chretien S, Mattei MG, Romeo PH (Jul 1991). A T-cell specific TCR delta DNA binding protein is a member of the human GATA family 2. Kouros-Mehr Hosein, Slorach EM, Sternlicht MD, Werb Z (December 2006). GATA-3 maintains the differentiation of the luminal cell fate in the mammary gland. Cell 127 (5): 1041–55. 3. Wilson BJ (2008). Does GATA3 act in tissue-specific pathways? A meta-analysis-based approach. J. Carcinogenesis 7: 6.

Uniprot ID: P23771

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 50 kDa

Calculated Molecular Weight:

Gene ID: 2625

Protein Name: Trans-acting T-cell-specific transcription factor GATA-3

Gene Full Name: GATA binding protein 3

Synonyms: GATA binding factor 3; GATA binding protein 3; GATA3; HDR