Anti-FHL2 Antibody Picoband®

SKU: A02129-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: FHL2

Specificity:

Background: Four and a half LIM domains protein 2 also known as FHL-2 is a protein that in humans is encoded by the FHL2 gene. This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. This protein is thought to have a role in the assembly of extracellular membranes. Also, this gene is down-regulated during transformation of normal myoblasts to rhabdomyosarcoma cells and the encoded protein may function as a link between presenilin-2 and an intracellular signaling pathway. Multiple alternatively spliced variants encoding different isoforms have been identified.

Immunogen: E.coli-derived human FHL2 recombinant protein (Position: T234-I279).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Amaar, Y. G., Thompson, G. R., Linkhart, T. A., Chen, S.-T., Baylink, D. J., Mohan, S. Insulin-like growth factor-binding protein 5 (IGFBP-5) interacts with a four and a half LIM protein 2 (FHL2). J. Biol. Chem. 277: 12053-12060, 2002. 2. Bai, S., Kitaura, H., Zhao, H., Chen, J., Muller, J. M., Schule, R., Darnay, B., Novack, D. V., Ross, F. P., Teitelbaum, S. L. FHL2 inhibits the activated osteoclast in a TRAF6-dependent manner. J. Clin. Invest. 115: 2742-2751, 2005. 3. Chan, K. K., Tsui, S. K. W., Lee, S. M. Y., Luk, S. C. W., Liew, C. C., Fung, K. P., Waye, M. M. Y., Lee, C. Y. Molecular cloning and characterization of FHL2, a novel LIM domain protein preferentially expressed in human heart. Gene 210: 345-350, 1998.

Uniprot ID: Q14192

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 32 kDa

Calculated Molecular Weight: 51990 MW

Gene ID: 2274

Protein Name: Four and a half LIM domains protein 2

Gene Full Name: four and a half LIM domains 2

Synonyms: AAG11; DRAL; FHL 2; FHL2; four and a half LIM domains 2; LIM domain protein DRAL; Skeletal muscle LIM protein 3; SLIM 3; SLIM3