Anti-Exonuclease 1/EXO1 Antibody Picoband®

SKU: A00536-3

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,ICC,IF,IP,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Immunoprecipitation, 0.5-2 μg/ml, Human
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: EXO1

Specificity:

Background: Exonuclease 1is anenzymethat in humans is encoded by theEXO1gene. It is mapped to 1q43. This gene encodes a protein with 5' to 3' exonuclease activity as well as an RNase H activity. It is similar to the Saccharomyces cerevisiae protein Exo1 which interacts with Msh2 and which is involved in mismatch repair and recombination. Alternative splicing of this gene results in three transcript variants encoding two different isoforms.

Immunogen: E.coli-derived human Exonuclease 1/EXO1 recombinant protein (Position: Q25-Q846).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1.Zakharyevich K, Tang S, Ma Y, Hunter N (2012). Delineation of joint molecule resolution pathways in meiosis identifies a crossover-specific resolvase. Cell. 149 (2): 334–47. 2. Wilson DM III, Carney JP, Coleman MA, Adamson AW, Christensen M, Lamerdin JE (September 1998). Hex1: a new human Rad2 nuclease family member with homology to yeast exonuclease 1. Nucleic Acids Res. 26 (16): 3762–8. doi:10.1093/nar/26.16.3762. 3. Schmutte C, Marinescu RC, Sadoff MM, Guerrette S, Overhauser J, Fishel R (November 1998). Human exonuclease I interacts with the mismatch repair protein hMSH2. Cancer Res. 58 (20): 4537–42.

Uniprot ID: Q9UQ84

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 120 kDa

Calculated Molecular Weight: 94 kDa

Gene ID: 9156

Protein Name: Exonuclease 1

Gene Full Name: exonuclease 1

Synonyms: EXO1; EXOI; exonuclease 1; Exonuclease I; HEX1; hExo1; hExoI