Anti-ETFA Antibody Picoband®

SKU: A05572-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,Flow Cytometry,IF,IHC,ICC,WB

Application Details: Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: ETFA

Specificity:

Background: The human ETFA gene encodes the Electron-transfer-flavoprotein, alpha subunit, also known as ETF-α. Together with Electron-transfer-flavoprotein, beta subunit, encoded by the 'ETFB' gene, it forms the heterodimericElectron transfer flavoprotein (ETF). ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene.

Immunogen: E.coli-derived human ETFA recombinant protein (Position: M1-K333).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Barton, D. E., Yang-Feng, T. L., Finocchiaro, G., Ozasa, H., Tanaka, K., Francke, U. Short chain acyl-CoA dehydrogenase (ACADS) maps to chromosome 12 (q22-qter) and electron transfer flavoprotein (ETFA) to 15 (q23-q25). (Abstract) Cytogenet. Cell Genet. 46: 577-578, 1987. 2. Finocchiaro, G., Ikeda, Y., Barton, D., Francke, U., Tanaka, K. Molecular cloning and gene mapping of alpha-subunit of human electron transfer flavoprotein. (Abstract) Am. J. Hum. Genet. 41: A214, 1987. 3. Finocchiaro, G., Ito, M., Ikeda, Y., Tanaka, K. Molecular cloning and nucleotide sequence of cDNAs encoding the alpha-subunit of human electron transfer flavoprotein. J. Biol. Chem. 263: 15773-15780, 1988.

Uniprot ID: P13804

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 35 kDa

Calculated Molecular Weight: 48608 MW

Gene ID: 2108

Protein Name: Electron transfer flavoprotein subunit alpha, mitochondrial

Gene Full Name: electron transfer flavoprotein subunit alpha

Synonyms: Alpha ETF; EMA; ETFA; GA2; MADD