Anti-EIF4E Monoclonal Antibody

SKU: M00135

Size: 100 μl

Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Form: Liquid

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,IP,Flow Cytometry

Application Details: WB 1:1000-5000
IHC 1:50-200
IP 1:30
FC 1:40

Gene Name: EIF4E

Specificity:

Background:

Immunogen: A synthesized peptide derived from human EIF4E eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types.

Clonality: Monoclonal

Contents: Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Affinity-chromatography

Reconstitution:

Reference:

Uniprot ID: P06730

Host: Rabbit

Concentration: 0.5mg/ml

Conjugate:

Cross Reactivity:

Isotype: Rabbit IgG

Phospho_site:

Clone Number: Clone: AFHI-5

Observed Molecular Weight: 25 kDa

Calculated Molecular Weight: 82785 MW

Gene ID: 1977

Protein Name: Eukaryotic translation initiation factor 4E

Gene Full Name: eukaryotic translation initiation factor 4E

Synonyms: CBP; CBP; EIF4E; eIF 4E; eIF 4F 25 kDa subunit; EIF4E; EIF4E1; EIF4EL1; EIF4F; mRNA cap binding protein