Anti-EHD1 Antibody Picoband®

SKU: A02168-2

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Monkey

Applications: Flow Cytometry,IF,ICC,WB

Application Details: Western blot, 0.25-0.5μg/ml, Human, Mouse, Monkey
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human

Gene Name: EHD1

Specificity:

Background: EH domain-containing protein 1, also known as testilin or PAST homolog 1 (PAST1), is a protein that in humans is encoded by the EHD1 gene, belonging to the EHD protein family. It is mapped to 11q13.1. This gene belongs to a highly conserved gene family encoding EPS15 homology (EH) domain-containing proteins. The protein-binding EH domain was first noted in EPS15, a substrate for the epidermal growth factor receptor. The EH domain has been shown to be an important motif in proteins involved in protein-protein interactions and in intracellular sorting. The protein encoded by this gene is thought to play a role in the endocytosis of IGF1 receptors. Alternatively spliced transcript variants have been found for this gene.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human EHD1, which shares 95% amino acid (aa) sequence identity with both mouse and rat EHD1.

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Haider, N. B., Searby, C., Galperin, E., Mintz, L., Horowitz, M., Stone, E. M., Sheffield, V. C. Evaluation and molecular characterization of EHD1, a candidate gene for Bardet-Biedl syndrome 1 (BBS1). Gene 240: 227-232, 1999. 2. Mintz, L., Galperin, E., Pasmanik-Chor, M., Tulzinsky, S., Bromberg, Y., Kozak, C. A., Joyner, A., Fein, A., Horowitz, M. EHD1--an EH-domain-containing protein with a specific expression pattern. Genomics 59: 66-76, 1999. 3. Naslavsky, N., Boehm, M., Backlund, P. S., Jr., Caplan, S. Rabenosyn-5 and EHD1 interact and sequentially regulate protein recycling to the plasma membrane. Molec. Biol. Cell 15: 2410-2422, 2004.

Uniprot ID: Q9H4M9

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 61 kDa

Calculated Molecular Weight: 63692 MW

Gene ID: 10938

Protein Name: EH domain-containing protein 1

Gene Full Name: EH domain containing 1

Synonyms: EH domain containing 1; EH domain containing protein 1; EHD1; H PAST; HPAST1; PAST; PAST homolog 1; PAST1; Testilin