Anti-DKK1 Antibody Picoband®

SKU: A00632

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB

Application Details: Western blot, 0.1-0.5μg/ml

Gene Name: DKK1

Specificity: No cross reactivity with other proteins.

Background: DKK1, Dickkopf-related protein 1, is a protein that in humans is encoded by the DKK1 gene. This gene encodes a protein that is a member of the dickkopf family. By genomic sequence analysis, determined that the DKK1 gene contains 4 exons. The DKK1 gene is mapped to 10q11.2 using FISH. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.

Immunogen: E. coli-derived human DKK1 recombinant protein (Position: T32-H266).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Fedi, P., Bafico, A., Soria, A. N., Burgess, W. H., Miki, T., Bottaro, D. P., Kraus, M. H., Aaronson, S. A.Isolation and biochemical characterization of the human Dkk-1 homologue, a novel inhibitor of mammalian Wnt signaling.J. Biol. Chem. 274: 19465-19472, 1999. 2. Roessler, E., Du, Y., Glinka, A., Dutra, A., Niehrs, C., Muenke, M.The genomic structure, chromosome location, and analysis of the human DKK1 head inducer gene as a candidate for holoprosencephaly. Cytogenet. Cell Genet. 89: 220-224, 2000. 3. Tian, E., Zhan, F., Walker, R., Rasmussen, E., Ma, Y., Barlogie, B., Shaughnessy, J. D., Jr.The role of Wnt-signaling antagonist DKK1 in the development of osteolytic lesions in multiple myeloma.New Eng. J. Med. 349: 2483-2494, 2003.

Uniprot ID: O94907

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 38 kDa

Calculated Molecular Weight:

Gene ID: 22943

Protein Name: Dickkopf-related protein 1

Gene Full Name: dickkopf Wnt signaling pathway inhibitor 1

Synonyms: Dickkopf 1; Dickkopf related protein 1; DKK 1; DKK1; hDkk 1; SK