Anti-DAZL Picoband® Antibody

SKU: A02069-2

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human, Mouse
ELISA, 0.1-0.5μg/ml, -

Gene Name: DAZL

Specificity: No cross reactivity with other proteins.

Background: Deleted in azoospermia-like is a protein that in humans is encoded by the DAZL gene. It is mapped to 3p24.3. The DAZ (Deleted in AZoospermia) gene family encodes potential RNA binding proteins that are expressed in prenatal and postnatal germ cells of males and females. The protein encoded by this gene is localized to the nucleus and cytoplasm of fetal germ cells and to the cytoplasm of developing oocytes. In the testis, this protein is localized to the nucleus of spermatogonia but relocates to the cytoplasm during meiosis where it persists in spermatids and spermatozoa. Transposition and amplification of this autosomal gene during primate evolution gave rise to the DAZ gene cluster on the Y chromosome. Mutations in this gene have been linked to severe spermatogenic failure and infertility in males. Two transcript variants encoding different isoforms have been found for this gene.

Immunogen: E.coli-derived human DAZL recombinant protein (Position: E34-R281).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Bartoloni, L., Cazzadore, C., Ferlin, A., Garolla, A., Foresta, C. Lack of the T54A polymorphism of the DAZL gene in infertile Italian patients. Molec. Hum. Reprod. 10: 613-615, 2004. 2. Eberhart, C. G., Maines, J. Z., Wasserman, S. A. Meiotic cell cycle requirement for a fly homologue of human Deleted in Azoospermia. Nature 381: 783-785, 1996. 3. Kee, K., Angeles, V. T., Flores, M., Nguyen, H. N., Reijo Pera, R. A. Human DAZL, DAZ and BOULE genes modulate primordial germ-cell and haploid gamete formation. Nature 462: 222-225, 2009.

Uniprot ID: Q92904

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 38 kDa

Calculated Molecular Weight:

Gene ID: 1618

Protein Name: Deleted in azoospermia-like

Gene Full Name: deleted in azoospermia like

Synonyms: DAZ homolog; DAZ like autosomal; DAZH; DAZL; DAZL1; DAZLA; deleted in azoospermia like; Deleted in azoospermia like 1; SPGY like autosomal; SPGYLA