Anti-Cytokeratin 14/KRT14 Antibody Picoband®

SKU: A01432

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: Flow Cytometry,IHC,IHC-F,ICC,WB

Application Details: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human
Immunocytochemistry, 0.5-1μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human

Gene Name: KRT14

Specificity: No cross reactivity with other proteins.

Background: Cytokeratin 14, also known as cytokeratin-14 (CK-14) or keratin-14 (KRT14), is a member of the type I keratin family of intermediate filament proteins. In humans it is encoded by the KRT14 gene. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Cytokeratin 14, identical to the related mouse and rat sequences.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Batta, K., Rugg, E. L., Wilson, N. J., West, N., Goodyear, H., Lane, E. B., Gratian, M., Dopping-Hepenstal, P., Moss, C., Eady, R. A. J. A keratin 14 'knockout' mutation in recessive epidermolysis bullosa simplex resulting in less severe disease. Brit. J. Derm. 143: 621-627, 2000. 2. Chan, Y., Anton-Lamprecht, I., Yu, Q.-C., Jackel, A., Zabel, B., Ernst, J.-P., Fuchs, E. A human keratin 14 'knockout': the absence of K14 leads to severe epidermolysis bullosa simplex and a function for an intermediate filament protein. Genes Dev. 8: 2574-2587, 1994. 3. Chen, H., Bonifas, J. M., Mstsumura, K., Ikeda, S., Leyden, W. A., Epstein, E. H. Keratin 14 gene mutations in patients with epidermolysis bullosa simplex. J. Invest. Derm. 105: 629-632, 1995.

Uniprot ID: P02533

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 60 kDa

Calculated Molecular Weight: 51561 MW

Gene ID: 3861

Protein Name: Keratin, type I cytoskeletal 14

Gene Full Name: keratin 14

Synonyms: Cell and organelle markers; CK 14; CK14; Cytokeratin 14; Cytoskeleton Marker; EBS3; EBS4; K14; keratin 14; KRT14; NFJ