Anti-Cytochrome P450 2E1/CYP2E1 Antibody Picoband®

SKU: PA1909

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Mouse,Rat

Applications: IHC,WB

Application Details: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat
Western blot, 0.1-0.5μg/ml, Mouse, Rat

Gene Name: CYP2E1

Specificity: No cross reactivity with other proteins.

Background: CYP2E1, also known as P450IIE1, is a member of the P450IIE subfamily which is ethanol-inducible. It has at least 1 gene which is mapped to 10q24.3-qter, and a second is likely in rat and in man. Both the rat and human proteins encoded by this gene contain 493 amino acids and calculated molecular masses of 56,634 and 56,916 daltons, respectively. In addition, genetic polymorphisms in the 5-prime flanking region of the human P450IIE1 gene affected its binding of transacting factor and changed its transcriptional regulation, which may lead to interindividual differences of microsomal drug oxidation activity. P450IIE1 is an important enzyme for the catalysis of the conversion of ethanol to acetaldehyde and to acetate in humans,and it is also involved in the metabolism of nitrosamines. Due to the possible correlation of P450IIE1 genes with malignancy, clinical studies of RFLP patterns of these genes in cancer may be useful.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of mouse CYP2E1, identical to the related rat sequence.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Nebert, D. W.; Adesnik, M.; Coon, M. J.; Estabrook, R. W.; Gonzalez, F. J.; Guengerich, F. P.; Gunsalus, I. C.; Johnson, E. F.; Kemper, B.; Levin, W.; Phillips, I. R.; Sato, R.; Waterman, M. R. : The P450 gene superfamily: recommended nomenclature. DNA 6: 1-11, 1987. 2. Song, B.-J.; Gelboin, H. V.; Park, S.-S.; Yang, C. S.; Gonzalez, F. J. : Complementary DNA and protein sequences of ethanol-inducible rat and human cytochrome P-450s: transcriptional and post-transcriptional regulation of the rat enzyme. J. Biol. Chem. 261: 16689-16697, 1987. 3. Hayashi, S.; Watanabe, J.; Kawajiri, K. : Genetic polymorphisms in the 5-prime-flanking region change transcriptional regulation of the human cytochrome P450IIE1 gene. J. Biochem. 110: 559-565, 1991.

Uniprot ID: Q05421

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 56 kDa

Calculated Molecular Weight: 56 kDa

Gene ID: 13106

Protein Name: Cytochrome P450 2E1

Gene Full Name: cytochrome P450, family 2, subfamily e, polypeptide 1

Synonyms: 4 nitrophenol 2 hydroxylase; CPE1; CYP2E; CYP2E1; CYP-2E1; CYP2E1-Specific; CYPIIE1; Cytochrome P450 2E1; Cytochrome P450 J; P450 J; P450C2E