Anti-Cyclin T1 Antibody Picoband® (monoclonal, 3B7)

SKU: M02703

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Monkey

Applications: WB,Flow Cytometry

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Monkey
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Gene Name: CCNT1

Specificity:

Background: Cyclin-T1 is a protein that in humans is encoded by the CCNT1 gene. This gene encodes a member of the highly conserved cyclin C subfamily. The encoded protein tightly associates with cyclin-dependent kinase 9, and is a major subunit of positive transcription elongation factor b (p-TEFb). In humans, there are multiple forms of positive transcription elongation factor b, which may include one of several different cyclins along with cyclin-dependent kinase 9. The complex containing the encoded cyclin and cyclin-dependent kinase 9 acts as a cofactor of human immunodeficiency virus type 1 (HIV-1) Tat protein, and is both necessary and sufficient for full activation of viral transcription. This cyclin and its kinase partner are also involved in triggering transcript elongation through phosphorylation of the carboxy-terminal domain of the largest RNA polymerase II subunit. Overexpression of this gene is implicated in tumor growth. Alternative splicing results in multiple transcript variants.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Cyclin T1, different from the related mouse sequence by one amino acid.

Clonality: Monoclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Entrez Gene: CCNT1 cyclin T1. 2. Peng J, Zhu Y, Milton JT, Price DH (April 1998). Identification of multiple cyclin subunits of human P-TEFb. Genes Dev. 12 (5): 755–62. 3. Wei P, Garber ME, Fang SM, Fischer WH, Jones KA (March 1998). A novel CDK9-associated C-type cyclin interacts ly with HIV-1 Tat and mediates its high-affinity, loop-specific binding to TAR RNA. Cell92 (4): 451–62.

Uniprot ID: O60563

Host: Mouse

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Mouse IgG2b

Phospho_site:

Clone Number: Clone: 3B7

Observed Molecular Weight: 81 kDa

Calculated Molecular Weight:

Gene ID: 904

Protein Name: Cyclin-T1

Gene Full Name: cyclin T1

Synonyms: CCNT; CCNT1; Cyclin T; cyclin T1; CYCT1; HIVE1