Anti-CPT1A Antibody Picoband®

SKU: A00917-3

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: CPT1A

Specificity:

Background: The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Immunogen: E.coli-derived human CPT1A recombinant protein (Position: M122-K556).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Andersen, M. K., Hansen, T. Genetics of metabolic traits in Greenlanders: lessons from an isolated population. J. Intern. Med. 284: 464-477, 2018. 2. Andersen, M. K., Jorsboe, E., Sandholt, C. H., Grarup, N., Jorgensen, M. E., Faergeman, N. J., Bjerregaard, P., Pedersen, O., Moltke, I., Hansen, T., Albrechtsen, A. Identification of novel genetic determinants of erythrocyte membrane fatty acid composition among Greenlanders. PLoS Genet. 12: e1006119, 2016. Note: Electronic Article. 3. Bergstrom, J. P., Reitz, R. C. Studies on carnitine palmitoyl transferase: the similar nature of CPTi (inner form) and CPTo (outer form). Arch. Biochem. Biophys. 204: 71-78, 1980.

Uniprot ID: P50416

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 88 kDa

Calculated Molecular Weight:

Gene ID: 1374

Protein Name: Carnitine O-palmitoyltransferase 1, liver isoform

Gene Full Name: carnitine palmitoyltransferase 1A

Synonyms: CPT I; CPT1; CPT1 L; CPT1A; CPTI L; L CPT1