Anti-CIT Antibody Picoband®

SKU: A01331-2

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: CIT

Specificity:

Background: This gene encodes a serine/threonine-protein kinase that functions in cell division. Together with the kinesin KIF14, this protein localizes to the central spindle and midbody, and functions to promote efficient cytokinesis. This protein is involved in central nervous system development. Polymorphisms in this gene are associated with bipolar disorder and risk for schizophrenia. Alternative splicing results in multiple transcript variants.

Immunogen: E.coli-derived human CIT recombinant protein (Position: Q470-H1505). Human CIT shares 95.2% and 94.7% amino acid (aa) sequence identity with mouse and rat CIT, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Basit, S., Al-Harbi, K. M., Alhijji, S. A. M., Albalawi, A. M., Alharby, E., Eldardear, A., Samman, M. I. CIT, a gene involved in neurogenic cytokinesis, is mutated in human primary microcephaly. Hum. Genet. 135: 1199-1207, 2016. 2. Di Cunto, F., Calautti, E., Hsiao, J., Ong, L., Topley, G., Turco, E., Dotto, G. P. Citron Rho-interacting kinase, a novel tissue-specific ser/thr kinase encompassing the Rho-Rac-binding protein citron. J. Biol. Chem. 273: 29706-29711, 1998. 3. Di Cunto, F., Imarisio, S., Hirsch, E., Broccoli, V., Bulfone, A., Migheli, A., Atzori, C., Turco, E., Triolo, R., Dotto, G. P., Silengo, L., Altruda, F. Defective neurogenesis in citron kinase knockout mice by altered cytokinesis and massive apoptosis. Neuron 28: 115-127, 2000.

Uniprot ID: O14578

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 231 kDa

Calculated Molecular Weight: 231 kDa

Gene ID: 11113

Protein Name: Citron Rho-interacting kinase

Gene Full Name: citron rho-interacting serine/threonine kinase

Synonyms: CIT; Citron Rho interacting kinase; CRIK; KIAA0949; STK21