Anti-cGKI/PRKG1 Antibody Picoband®

SKU: A01708-3

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,Flow Cytometry,IF,IHC,ICC,WB

Application Details: Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human, Rat
ELISA, 0.1-0.5μg/ml, -

Gene Name: PRKG1

Specificity:

Background: cGMP-dependent protein kinase 1, alpha isozyme is an enzyme that in humans is encoded by the PRKG1 gene. Mammals have three different isoforms of cyclic GMP-dependent protein kinase (Ialpha, Ibeta, and II). These PRKG isoforms act as key mediators of the nitric oxide/cGMP signaling pathway and are important components of many signal transduction processes in diverse cell types. This PRKG1 gene on human chromosome 10 encodes the soluble Ialpha and Ibeta isoforms of PRKG by alternative transcript splicing. A separate gene on human chromosome 4, PRKG2, encodes the membrane-bound PRKG isoform II. The PRKG1 proteins play a central role in regulating cardiovascular and neuronal functions in addition to relaxing smooth muscle tone, preventing platelet aggregation, and modulating cell growth. This gene is most strongly expressed in all types of smooth muscle, platelets, cerebellar Purkinje cells, hippocampal neurons, and the lateral amygdala. Isoforms Ialpha and Ibeta have identical cGMP-binding and catalytic domains but differ in their leucine/isoleucine zipper and autoinhibitory sequences and therefore differ in their dimerization substrates and kinase enzyme activity.

Immunogen: E.coli-derived human cGKI/PRKG1 recombinant protein (Position: S2-Q44).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Burgoyne, J. R., Madhani, M., Cuello, F., Charles, R. L., Brennan, J. P., Schroder, E., Browning, D. D., Eaton, P. Cysteine redox sensor in PKGI-alpha enables oxidant-induced activation. Science 317: 1393-1397, 2007. 2. Foller, M., Feil, S., Ghoreschi, K., Koka, S., Gerling, A., Thunemann, M., Hofmann, F., Schuler, B., Vogel, J., Pichler, B., Kasinathan, R. S., Nicolay, J. P., Huber, S. M., Lang, F., Feil, R. Anemia and splenomegaly in cGKI-deficient mice. Proc. Nat. Acad. Sci. 105: 6771-6776, 2008. 3. Guo, D., Regalado, E., Casteel, D. E., Santos-Cortez, R. L., Gong, L., Kim, J. J., Dyack, S., Horne, S. G., Chang, G., Jondeau, G., Boileau, C., Coselli, J. S., and 10 others. Recurrent gain-of-function mutation in PRKG1 causes thoracic aortic aneurysms and acute aortic dissections. Am. J. Hum. Genet. 93: 398-404, 2013.

Uniprot ID: Q13976

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 78 kDa

Calculated Molecular Weight:

Gene ID: 5592

Protein Name: cGMP-dependent protein kinase 1

Gene Full Name: protein kinase cGMP-dependent 1

Synonyms: cGK 1; cGK1; CGKI; cGKI alpha; cGKI BETA; PGK; PKG; PRKG1; PRKG1B; PRKGR1A; PRKGR1B; Protein Kinase G1 alpha