Anti-CEP135 Antibody Picoband®

SKU: A06361-4

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/ml

Gene Name: CEP135

Specificity:

Background: Centrosomal protein of 135 kDa is a protein that in humans is encoded by the CEP135 gene. This gene encodes a centrosomal protein, which acts as a scaffolding protein during early centriole biogenesis, and is also required for centriole-centriole cohesion during interphase. Mutations in this gene are associated with autosomal recessive primary microcephaly-8.

Immunogen: E.coli-derived human CEP135 recombinant protein (Position: E28-E1034). Human CEP135 shares 82.8% amino acid (aa) sequence identity with mouse CEP135.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Andersen, J. S., Wilkinson, C. J., Mayor, T., Mortensen, P., Nigg, E. A., Mann, M. Proteomic characterization of the human centrosome by protein correlation profiling. Nature 426: 570-574, 2003. 2. Carvalho-Santos, Z., Machado, P., Branco, P., Tavares-Cadete, F., Rodrigues-Martins, A., Pereira-Leal, J. B., Bettencourt-Dias, M. Stepwise evolution of the centriole-assembly pathway. J. Cell Sci. 123: 1414-1426, 2010. 3. Farooq, M., Fatima, A., Mang, Y., Hansen, L., Kjaer, K. W., Baig, S. M., Larsen, L. A., Tommerup, N. A novel splice site mutation in CEP135 is associated with primary microcephaly in a Pakistani family. J. Hum. Genet. 61: 271-273, 2016.

Uniprot ID: Q66GS9

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 133 kDa

Calculated Molecular Weight: 133 kDa

Gene ID: 9662

Protein Name: Centrosomal protein of 135 kDa

Gene Full Name: centrosomal protein 135

Synonyms: centrosomal protein 135kDa; Centrosomal protein 4; Centrosomal protein of 135 kDa; CEP135; CEP4; KIAA0635