Anti-CD326/Epcam Antibody Picoband®

SKU: A00276-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Mouse,Rat

Applications: ELISA,Flow Cytometry,IF,IHC,WB

Application Details: Western blot, 0.1-0.25 μg/ml, Mouse
Immunohistochemistry(Paraffin-embedded Section), 1-2 μg/ml, Mouse, Rat
Immunofluorescence, 5 μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg /1x10⁶ cells, Mouse
ELISA, 0.1-0.5 μg/ml, -

Gene Name: Epcam

Specificity:

Background: Epithelial cell adhesion molecule (EpCAM) is a transmembrane glycoprotein mediating Ca2+-independent homotypic cell–cell adhesion in epithelia. This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy.

Immunogen: E.coli-derived mouse CD326/Epcam recombinant protein (Position: Q24-A267).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Litvinov, Sergey; et al. (1994). Ep-CAM: a human epithelial antigen is a homophilic cell–cell adhesion molecule. The Journal of Cell Biology 125 (2): 437–46. 2. Maetzel, Dorothea; et al. (2009). Nuclear signalling by tumour-associated antigen EpCAM. Nature Cell Biology 11 (2): 162–71.

Uniprot ID: Q99JW5

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 38 kDa

Calculated Molecular Weight: 68762 MW

Gene ID: 17075

Protein Name: Epithelial cell adhesion molecule

Gene Full Name: epithelial cell adhesion molecule

Synonyms: CD326; CO 17A; CO17 1A; EGP; EGP 2; EGP314; EGP34; EGP40; Ep CAM; EPCAM; Epithelial glycoprotein; Epithelial glycoprotein 314; ESA; GA733 2; hEGP 2; hEGP314; KS 1/4 antigen; KS1/4; KSA; M1S2; M4S1; MIC18; MK 1; TACST 1; TACSTD1; TROP1