Anti-Caseine Kinase 1 alpha/CSNK1A1 Antibody Picoband®

SKU: PB9693

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: IHC,WB

Application Details: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Gene Name: CSNK1A1

Specificity: No cross reactivity with other proteins.

Background: Casein kinase I isoform alpha?is an?enzyme?that in humans is encoded by the?CSNK1A1?gene. The?CSNK1A1?gene is mapped to chromosome 5q32 based on an alignment of the?CSNK1A1?sequence with the genomic sequence (GRCh37). It is reported that both screens identified CK1-alpha as a bifunctional regulator of NF-kappa-B. CK1-alpha dynamically associates with the CBM complex on T cell receptor engagement to participate in cytokine production and lymphocyte proliferation. However, CK1-alpha kinase activity has a contrasting role by subsequently promoting the phosphorylation and inactivation of CARMA1. CK1-alpha has thus a dual 'gating' function which first promotes and then terminates receptor-induced NF-kappa-B. ABC DLBCL cells required CK1-alpha for constitutive NF-kappa-B activity, indicating that CK1-alpha functions as a conditionally essential malignancy gene.?

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human CSNK1A1, identical to the related mouse and rat sequences.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Bidere, N., Ngo, V. N., Lee, J., Collins, C., Zheng, L., Wan, F., Davis, R. E., Lenz, G., Anderson, D. E., Arnoult, D., Vazquez, A., Sakai, K., Zhang, J., Meng, Z., Veenstra, T. D., Staudt, L. M., Lenardo, M. J. Casein kinase 1-alpha governs antigen-receptor-induced NF-kappa-B activation and human lymphoma cell survival. Nature 458: 92-96, 2009. 2. Elyada, E., Pribluda, A., Goldstein, R. E., Morgenstern, Y., Brachya, G., Cojocaru, G., Snir-Alkalay, I., Burstain, I., Haffner-Krausz, R., Jung, S., Wiener, Z., Alitalo, K., Oren, M., Pikarsky, E., Ben-Neriah, Y. CKI-alpha ablation highlights a critical role for p53 in invasiveness control. Nature 470: 409-413, 2011. 3. Tapia C, Featherstone T, Gómez C, Taillon-Miller P, Allende CC, Allende JE (Aug 1994). Cloning and chromosomal localization of the gene coding for human protein kinase CK1. FEBS Letters 349 (2): 307–12.

Uniprot ID: P48729

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 39 kDa

Calculated Molecular Weight: 38915 MW

Gene ID: 1452

Protein Name: Casein kinase I isoform alpha

Gene Full Name: casein kinase 1 alpha 1

Synonyms: casein kinase 1; alpha 1; Casein kinase I isoform alpha; CK1; CKI alpha; CSNK1A1; HLCDGP1; PRO2975