Anti-Calmegin/Clgn Antibody Picoband®

SKU: A05261-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Mouse,Rat

Applications: WB,ELISA

Application Details: Western blot, 0.1-0.25 μg/ml, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -

Gene Name: Clgn

Specificity:

Background: Calmegin, also known as CLGN, is a protein which in humans is encoded by the CLGN gene. This gene belongs to the calreticulin family, which includes calreticulin, calnexin, and calmegin, and encodes a calcium-binding molecular chaperone specifically expressed in pachytene stage male germ cells. It is required for the proper folding of newly synthesized membrane proteins in the endoplasmic reticulum including those critical for sperm migration from the uterus into the oviduct and sperm adhesion to and penetration of the zona pellucida. This gene plays a key role in spermatogenesis and male infertility. Alternative splice variants exist for this gene.

Immunogen: E.coli-derived mouse Calmegin/Clgn recombinant protein (Position: N18-D611).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Ikawa, M., Nakanishi, T., Yamada, S., Wada, I., Kominami, K., Tanaka, H., Nozaki, M., Nishimune, Y., Okabe, M. Calmegin is required for fertilin alpha/beta heterodimerization and sperm fertility. Dev. Biol. 240: 254-261, 2001. 2. Ikawa, M., Wada, I., Kominami, K., Watanabe, D., Toshimori, K., Nishimune, Y., Okabe, M. The putative chaperone calmegin is required for sperm fertility. Nature 387: 607-611, 1997. 3. Tanaka, H., Ikawa, M., Tsuchida, J., Nozaki, M., Suzuki, M., Fujiwara, T., Okabe, M., Nishimune, Y. Cloning and characterization of the human calmegin gene encoding putative testis-specific chaperone. Gene 204: 159-163, 1997.

Uniprot ID: P52194

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 95 kDa

Calculated Molecular Weight: 39411 MW

Gene ID: 12745

Protein Name: Calmegin

Gene Full Name: calmegin

Synonyms: calmegin; CLGN