Anti-C3 Antibody Picoband®

SKU: A00168-3

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,Flow Cytometry,WB

Application Details: Western blot, 0.25-0.5μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
ELISA, 0.1-0.5μg/ml, -

Gene Name: C3

Specificity:

Background: Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form the mature protein, which is then further processed to generate numerous peptide products. The C3a peptide, also known as the C3a anaphylatoxin, modulates inflammation and possesses antimicrobial activity. Mutations in this gene are associated with atypical hemolytic uremic syndrome and age-related macular degeneration in human patients.

Immunogen: E.coli-derived human C3 recombinant protein (Position: Q1277-E1538). Human C3 shares 73.7 % and 76.3% amino acid (aa) sequence identity with mouse and rat C3, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Ajees, A. A., Gunasekaran, K., Volanakis, J. E., Narayana, S. V. L., Kotwal, G. J., Krishna Murthy, H. M. The structure of complement C3b provides insights into complement activation and regulation. Nature 444: 221-225, 2006. Note: Retraction: Nature 532: 268 only, 2016. 2. Alper, C. A., Colten, H. R., Gear, J. S. S., Rabson, A. R., Rosen, F. S. Homozygous human C3 deficiency: the role of C3 in antibody production, C1s-induced vasopermeability, and cobra venom-induced passive hemolysis. J. Clin. Invest. 57: 222-229, 1976. 3. Alper, C. A., Colten, H. R., Rosen, S. F., Rabson, A. R., MacNab, G. M., Gear, J. S. S. Homozygous deficiency of C3 in a patient with repeated infections. Lancet 300: 1179-1181, 1972. Note: Originally Volume II.

Uniprot ID: P01024

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 128 kDa, 187 kDa

Calculated Molecular Weight:

Gene ID: 718

Protein Name: Complement C3

Gene Full Name: complement C3

Synonyms: ARMD9; ASP; C3; C3b; C3c; Complement C3; complement component 3; CPAMD1