Anti-BCL6 Antibody Picoband®

SKU: A00142-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: BCL6

Specificity:

Background: Bcl-6 (B-cell lymphoma 6) is a protein that in humans is encoded by the BCL6 gene. The protein encoded by this gene is a zinc finger transcription factor and contains an N-terminal POZ domain. This protein acts as a sequence-specific repressor of transcription, and has been shown to modulate the transcription of STAT-dependent IL-4 responses of B cells. This protein can interact with a variety of POZ-containing proteins that function as transcription corepressors. This gene is found to be frequently translocated and hypermutated in diffuse large-cell lymphoma (DLCL), and may be involved in the pathogenesis of DLCL. Alternatively spliced transcript variants encoding different protein isoforms have been found for this gene.

Immunogen: E.coli-derived human BCL6 recombinant protein (Position: R28-K336). Human BCL6 shares 90.6% amino acid (aa) sequence identity with mouse BCL6.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Akasaka, T., Miura, I., Takahashi, N., Akasaka, H., Yonetani, N., Ohno, H., Fukuhara, S., Okuma, M. A recurring translocation, t(3;6)(q27;p21), in non-Hodgkin's lymphoma results in replacement of the 5-prime regulatory region of BCL6 with a novel H4 histone gene. Cancer Res. 57: 7-12, 1997. 2. Barnett, B. E., Ciocca, M. L., Goenka, R., Barnett, L. G., Wu, J., Laufer, T. M., Burkhardt, J. K., Cancro, M. P., Reiner, S. L. Asymmetric B cell division in the germinal center reaction. Science 335: 342-344, 2012. 3. Baron, B. W., Nucifora, G., McCabe, N., Espinosa, R., III, Le Beau, M. M., McKeithan, T. W. Identification of the gene associated with the recurring chromosomal translocations t(3;14)(q27;q32) and t(3;22)(q27;q11) in B-cell lymphomas. Proc. Nat. Acad. Sci. 90: 5262-5266, 1993.

Uniprot ID: P41182

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity:

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 88 kDa

Calculated Molecular Weight: 79 kDa

Gene ID: 604

Protein Name: B-cell lymphoma 6 protein

Gene Full Name: BCL6 transcription repressor

Synonyms: B cell CLL/lymphoma 6; B cell lymphoma 5 protein; B cell lymphoma 6 protein; BCL6; LAZ3; Protein LAZ 3; ZBTB27; Zinc finger protein 51; ZNF51