Anti-ATP6V1H Antibody Picoband®

SKU: A11008-3

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Mouse,Rat

Applications: WB,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Mouse, Rat
ELISA, 0.1-0.5 μg/ml

Gene Name: ATP6V1H

Specificity:

Background: This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular organelles. V-ATPase-dependent organelle acidification is necessary for multiple processes including protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. The encoded protein is the regulatory H subunit of the V1 domain of V-ATPase, which is required for catalysis of ATP but not the assembly of V-ATPase. Decreased expression of this gene may play a role in the development of type 2 diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Immunogen: E.coli-derived human ATP6V1H recombinant protein (Position: Q40-S483). Human ATP6V1Hshares 98.4% and 98% amino acid (aa) sequence identity with mouse and rat ATP6V1H, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Geyer, M., Fackler, O. T., Peterlin, B. M. Subunit H of the V-ATPase involved in endocytosis shows homology to beta-adaptins. Molec. Biol. Cell 13: 2045-2056, 2002. 2. Geyer, M., Yu, H., Mandic, R., Linnemann, T., Zheng, Y.-H., Fackler, O. T., Peterlin, B. M. Subunit H of the V-ATPase binds to the medium chain of adaptor protein complex 2 and connects Nef to the endocytic machinery. J. Biol. Chem. 277: 28521-28529, 2002. 3. Lu, X., Yu, H., Liu, S.-H., Brodsky, F. M., Peterlin, B. M. Interactions between HIV1 Nef and vacuolar ATPase facilitate the internalization of CD4. Immunity 8: 647-656, 1998.

Uniprot ID: Q9UI12

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 50 kDa

Calculated Molecular Weight: 56 kDa

Gene ID: 51606

Protein Name: V-type proton ATPase subunit H

Gene Full Name: ATPase H+ transporting V1 subunit H

Synonyms: ATP6V1H; CGI 11; MSTP042; NBP1; Nef binding protein 1; SFD; SFDalpha; SFDbeta; V ATPase 50/57 kDa subunits; V ATPase subunit H; V type proton ATPase subunit H; Vacuolar proton pump subunit H; VMA13