Anti-ATF1 Antibody Picoband®

SKU: PB9130

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: Flow Cytometry,ICC/IF,WB

Application Details: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human

Gene Name: ATF1

Specificity: No cross reactivity with other proteins.

Background: ATF1, also known as activating transcription factor 1, is a protein that in humans is encoded by the ATF1 gene. It is mapped to 12q13.12. This gene encodes an activating transcription factor, which belongs to the ATF subfamily and bZIP (basic-region leucine zipper) family. It influences cellular physiologic processes by regulating the expression of downstream target genes, which are related to growth, survival, and other cellular activities. This protein is phosphorylated at serine 63 in its kinase-inducible domain by serine/threonine kinases, cAMP-dependent protein kinase A, calmodulin-dependent protein kinase I/II, mitogen- and stress-activated protein kinase and cyclin-dependent kinase 3 (cdk-3). Its phosphorylation enhances its transactivation and transcriptional activities, and enhances cell transformation.

Immunogen: E.coli-derived human ATF1 recombinant protein (Position: M1-V271). Human ATF1 shares 91% amino acid (aa) sequence identity with mouse ATF1.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. ucman, J., Delattre, O., Desmaze, C., Epstein, A. L., Stenman, G., Speleman, F., Fletchers, C. D. M., Aurias, A., Thomas, G. EWS and ATF-1 gene fusion induced by t(12;22) translocation in malignant melanoma of soft parts. Nature Genet. 4: 341-345, 1993. 2. Entrez Gene: ATF1 activating transcription factor 1

Uniprot ID: P18846

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 35 kDa

Calculated Molecular Weight: 29 kDa

Gene ID: 466

Protein Name: Cyclic AMP-dependent transcription factor ATF-1

Gene Full Name: activating transcription factor 1

Synonyms: ATF1; EWS ATF1; FUS/ATF 1; Protein TREB36; TREB36