Anti-Apolipoprotein J/CLU Antibody Picoband®

SKU: PB9575

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: ELISA,IHC,WB

Application Details: ELISA, 0.1-0.5μg/ml, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Western blot, 0.1-0.5μg/ml, Human

Gene Name: CLU

Specificity: No cross reactivity with other proteins.

Background: Clusterin (apolipoprotein J) is a 75 - 80 kDa disulfide-linked heterodimeric protein associated with the clearance of cellular debris and apoptosis. In humans, clusterin is encoded by the CLU gene. This protein has several synonyms: dimeric acidic glycoprotein (DAG protein), testosterone repressed prostate message-2 (TRPM-2), sulfated glycoprotein-2 (SGP-2) and complement lysis inhibitor (CLI). Clusterin was mapped to mouse chromosome 14. Clusterin is a ubiquitously expressed molecule thought to influence a variety of processes including cell death. In the brain, it accumulates in dying neurons following seizures and hypoxic-ischemic (H-I) injury. clusterin may be a new therapeutic target to modulate non-caspase-dependent neuronal death following acute brain injury.

Immunogen: E. coli-derived human Apolipoprotein J recombinant protein (Position: S228-E449). Human Apolipoprotein J shares 76.1 % and 75.2 % amino acid (aa) sequence identity with mouse and rat Apolipoprotein J, respectively.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Entrez Gene: clusterin. 2. Birkenmeier, E. H., Letts, V. A., Frankel, W. N., Magenheimer, B. S., Calvet, J. P. Sulfated glycoprotein-2 (Sgp-2) maps to mouse chromosome 14. Mammalian Genome 4: 131-132, 1993. 3. Han, B. H., DeMattos, R. B., Dugan, L. L., Kim-Han, J. S., Brendza, R. P., Fryer, J. D., Kierson, M., Cirrito, J., Quick, K., Harmony, J. A. K., Aronow, B. J., Holtzman, D. M. Clusterin contributes to caspase-3-independent brain injury following neonatal hypoxia-ischemia. Nature Med. 7: 338-343, 2001. 4. Jones SE, Jomary C (May 2002). Clusterin. Int. J. Biochem. Cell Biol. 34 (5): 427–431.

Uniprot ID: P10909

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 82 kDa

Calculated Molecular Weight: 52495 MW

Gene ID: 1191

Protein Name: Clusterin

Gene Full Name: clusterin

Synonyms: AAG4; Apo J; APOJ; ApoJalpha; ApoJbeta; Apolipoprotein J; CLI; CLU; clusterin; Complement cytolysis inhibitor; Ku70 binding protein 1; KUB1; NA1/NA2; SGP 2; SGP2; SP 40; TRPM 2; TRPM2