Anti-AP2B1 Antibody Picoband®

SKU: A06544

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB

Application Details: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Gene Name: AP2B1

Specificity: No cross reactivity with other proteins.

Background: AP-2 complex subunit beta is a protein that in humans is encoded by the AP2B1 gene. It is mapped to 17q12. The protein encoded by this gene is one of two large chain components of the assembly protein complex 2, which serves to link clathrin to receptors in coated vesicles. The encoded protein is found on the cytoplasmic face of coated vesicles in the plasma membrane. Two transcript variants encoding different isoforms have been found for this gene.

Immunogen: E. coli-derived human AP2B1 recombinant protein (Position: Q311-R522). Human AP2B1 shares 100% amino acid (aa) sequence identity with both mouse and rat AP2B1.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Entrez Gene: AP2B1 adaptor-related protein complex 2, beta 1 subunit. 2. Druck T, Gu Y, Prabhala G, Cannizzaro LA, Park SH, Huebner K, Keen JH (Nov 1995). Chromosome localization of human genes for clathrin adaptor polypeptides AP2 beta and AP50 and the clathrin-binding protein, VCP. Genomics. 30 (1): 94–7. 3. Gallusser A, Kirchhausen T (Dec 1993). The beta 1 and beta 2 subunits of the AP complexes are the clathrin coat assembly components. The EMBO Journal. 12 (13): 5237–44.

Uniprot ID: P63010

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 105 kDa

Calculated Molecular Weight: 104553 MW

Gene ID: 163

Protein Name: AP-2 complex subunit beta

Gene Full Name: adaptor related protein complex 2 subunit beta 1

Synonyms: ADTB2; AP 2 complex subunit beta; AP105B; AP2 BETA; AP2B1; Beta 2 adaptin; Beta adaptin; CLAPB1