Anti-AP2 alpha/TFAP2A Antibody Picoband®

SKU: PB9118

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Rat

Applications: WB

Application Details: Western blot, 0.1-0.5μg/ml, Human, Rat

Gene Name: TFAP2A

Specificity: No cross reactivity with other proteins.

Background: TFAP2A, also known as AP-2alpha or BOFS, is a protein that in humans is encoded by the TFAP2A gene. It is mapped to 6p24.3. The protein encoded by this gene is a transcription factor that binds the consensus sequence 5'-GCCNNNGGC-3'. The encoded protein functions as either a homodimer or as a heterodimer with similar family members. This protein activates the transcription of some genes while inhibiting the transcription of others. TFAP2A acts as a sequence-specific DNA-binding transcription factor recognizing and binding to the specific DNA sequence and recruiting transcription machinery. This gene is expressed in neural crest cell lineages with the highest levels of expression corresponding to early neural crest cells, suggesting that TFAP2A plays a role in their differentiation and development.

Immunogen: E.coli-derived human AP2 alpha recombinant protein (Position: M1-G166). Human AP2 alpha shares 98% amino acid (aa) sequence identity with both mouse and rat AP2 alpha.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Williams T, Tjian R (April 1991). Analysis of the DNA-binding and activation properties of the human transcription factor AP-2. Genes & Development 5 (4): 670–82. 2. Mitchell PJ, Wang C, Tjian R (September 1987). Positive and negative regulation of transcription in vitro: enhancer-binding protein AP-2 is inhibited by SV40 T antigen. Cell 50 (6): 847–61. 3. Williams T, Admon A, Lüscher B, Tjian R (December 1988). Cloning and expression of AP-2, a cell-type-specific transcription factor that activates inducible enhancer elements. Genes & Development 2 (12A): 1557–69. 4. Le Douarin NM, Ziller C, Couly GF (September 1993). Patterning of neural crest derivatives in the avian embryo: in vivo and in vitro studies. Developmental Biology 159 (1): 24–49.

Uniprot ID: P05549

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 48 kDa

Calculated Molecular Weight: 48062 MW

Gene ID: 7020

Protein Name: Transcription factor AP-2-alpha

Gene Full Name: transcription factor AP-2 alpha

Synonyms: Activator protein 2; AP 2; AP 2 transcription factor; AP 2alpha; AP2; AP2 alpha; AP2TF; BOFS; TFAP2; TFAP2A; TFAP2A; AP-2