Anti-Alpha SNAP/NAPA Antibody Picoband®

SKU: A05153-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,Flow Cytometry

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Gene Name: NAPA

Specificity:

Background: N-ethylmaleimide-sensitive factor Attachment Protein Alpha, also known as SNAP-α, is a SNAP protein that is involved in the intra-cellular trafficking and fusing of vesicles to target membranes in cells. This gene encodes a member of the soluble NSF attachment protein (SNAP) family. SNAP proteins play a critical role in the docking and fusion of vesicles to target membranes as part of the 20S NSF-SNAP-SNARE complex. The encoded protein plays a role in the completion of membrane fusion by mediating the interaction of N-ethylmaleimide-sensitive factor (NSF) with the vesicle-associated and membrane-associated SNAP receptor (SNARE) complex, and stimulating the ATPase activity of NSF. Alternatively spliced transcript variants have been observed for this gene.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Alpha SNAP/NAPA, which shares 95.7% amino acid (aa) sequence identity with mouse and rat NAPA.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Andreeva, A. V., Kutuzov, M. A., Vaiskunaite, R., Profirovic, J., Meigs, T. E., Predescu, S., Malik, A. B., Voyno-Yasenetskaya, T. G-alpha-12 interaction with alpha-SNAP induces VE-cadherin localization at endothelial junctions and regulates barrier function. J. Biol. Chem. 280: 30376-30383, 2005. 2. Bronson, R. T., Lane, P. W. Hydrocephalus with hop gait (hyh): a new mutation on chromosome 7 in the mouse. Dev. Brain Res. 54: 131-136, 1990. 3. Chae, T. H., Allen, K. M., Davisson, M. T., Sweet, H. O., Walsh, C. A. Mapping of the mouse hyh gene to a YAC/BAC contig on proximal chromosome 7. Mammalian Genome 13: 239-244, 2002.

Uniprot ID: P54920

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 33 kDa

Calculated Molecular Weight: 67074 MW

Gene ID: 8775

Protein Name: Alpha-soluble NSF attachment protein

Gene Full Name: NSF attachment protein alpha

Synonyms: alpha-SNAP; NAPA; SNAP alpha; SNAPA