Anti-Alcohol dehydrogenase class-3 ADH5 Antibody Picoband®

SKU: RP1111

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: IF,IHC,ICC,WB

Application Details: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human

Gene Name: ADH5

Specificity: No cross reactivity with other proteins.

Background: Alcohol dehydrogenase class-3 is an enzyme that in humans is encoded by the ADH5 gene. This gene encodes a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. The encoded protein forms a homodimer. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long-chain primary alcohols and for oxidation of S-hydroxymethyl-glutathione, a spontaneous adduct between formaldehyde and glutathione. This enzyme is an important component of cellular metabolism for the elimination of formaldehyde, a potent irritant and sensitizing agent that causes lacrymation, rhinitis, pharyngitis, and contact dermatitis. The human genome contains several non-transcribed pseudogenes related to this gene.

Immunogen: E. coli-derived human ADH5 recombinant protein (Position: K212-I374). Human ADH5 shares 90.2% and 92% amino acid (aa) sequence identity with mouse and rat ADH5, respectively.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Adinolfi A, Adinolfi M, Hopkinson DA (May 1984). Immunological and biochemical characterization of the human alcohol dehydrogenase chi-ADH isozyme. Ann Hum Genet 48 (Pt 1): 1–10. 2. Hur MW, Edenberg HJ (Dec 1992). Cloning and characterization of the ADH5 gene encoding human alcohol dehydrogenase 5, formaldehyde dehydrogenase. Gene 121(2): 305–11.

Uniprot ID: P11766

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 39 kDa

Calculated Molecular Weight: 39724 MW

Gene ID: 128

Protein Name: Alcohol dehydrogenase class-3

Gene Full Name: alcohol dehydrogenase 5 (class III), chi polypeptide

Synonyms: ADH 3; ADH5; ADHX; Alcohol dehydrogenase 5; Alcohol dehydrogenase class 3; FALDH; FDH; GSH FDH; GSNOR; GSNOR; ADH5