Anti-Alanine Transaminase/Gpt Antibody Picoband®

SKU: A01638-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Mouse,Rat

Applications: ELISA,Flow Cytometry,IF,IHC,ICC,WB

Application Details: Western blot, 0.1-0.25 μg/ml, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse
ELISA, 0.1-0.5 μg/ml, -

Gene Name: Gpt

Specificity:

Background: This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase

Immunogen: E.coli-derived mouse Alanine Transaminase/Gpt recombinant protein (Position: A2-S496).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Astrin, K. H., Arredondo-Vega, F. X., Desnick, R. J., Smith, M. Assignment of the gene for cytosolic alanine aminotransferase (AAT1) to human chromosome 8. Ann. Hum. Genet. 46: 125-133, 1982. 2. Chen, S.-H., Giblett, E. R. Polymorphism of soluble glutamic-pyruvic transaminase: a new genetic marker in man. Science 173: 148-149, 1971. 3. Cook, P. J. L., Jeremiah, S. J., Buckton, K. E. Exclusion mapping of GPT. (Abstract) Cytogenet. Cell Genet. 32: 258 only, 1982.

Uniprot ID: Q8QZR5

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 55 kDa

Calculated Molecular Weight: 141435 MW

Gene ID: 76282

Protein Name: Alanine aminotransferase 1

Gene Full Name: glutamic pyruvic transaminase, soluble

Synonyms: AAT1; Alanine aminotransferase 1; ALAT1; ALT1; GPT; GPT 1; GPT/ ALT1; GPT1