Anti-AKAP12 Antibody Picoband®

SKU: A02303-3

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Mouse,Rat

Applications: ELISA,Flow Cytometry,IF,IHC,ICC,WB

Application Details: Western blot, 0.1-0.25μg/ml, Mouse
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse
Immunocytochemistry/Immunofluorescence, 5μg/ml, Mouse
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Mouse, Rat
ELISA, 0.1-0.5μg/ml, -

Gene Name: Akap12

Specificity:

Background: A-kinase anchor protein 12, aka AKAP250, is an enzyme that in humans is encoded by the AKAP12 gene. The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells. It associates with protein kinase A and C and phosphatase, and serves as a scaffold protein in signal transduction. This protein and RII PKA colocalize at the cell periphery. This protein is a cell growth-related protein. Antibodies to this protein can be produced by patients with myasthenia gravis. Alternative splicing of this gene results in two transcript variants encoding different isoforms.

Immunogen: E.coli-derived mouse AKAP12 recombinant protein (Position: E387-H1620).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Gordon, T., Grove, B., Loftus, J. C., O'Toole, T., McMillan, R., Lindstrom, J., Ginsberg, M. H. Molecular cloning and preliminary characterization of a novel cytoplasmic antigen recognized by myasthenia gravis sera. J. Clin. Invest. 90: 992-999, 1992. 2. Lee, S.-W., Kim, W. J., Choi, Y. K., Song, H. S., Son, M. J., Gelman, I. H., Kim, Y.-J., Kim, K.-W. SSeCKS regulates angiogenesis and tight junction formation in blood-brain barrier. Nature Med. 9: 900-906, 2003. 3. Nauert, J. B., Klauck, T. M., Langeberg, L. K., Scott, J. D. Gravin, an autoantigen recognized by serum from myasthenia gravis patients, is a kinase scaffold protein. Curr. Biol. 7: 52-62, 1997.

Uniprot ID: Q9WTQ5

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 300 kDa

Calculated Molecular Weight: 30392 MW

Gene ID: 83397

Protein Name: A-kinase anchor protein 12

Gene Full Name: A kinase anchor protein 12

Synonyms: A kinase anchor protein 12; AKAP 12; AKAP 250; AKAP12; AKAP250; Gravin; Myasthenia gravis autoantigen