Anti-5HT1B/HTR1B Antibody Picoband®

SKU: A01826-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: HTR1B

Specificity:

Background: 5-hydroxytryptamine receptor 1B also known as the 5-HT1B receptor is a protein that in humans is encoded by the HTR1B gene. The protein encoded by this intronless gene is a G-protein coupled receptor for serotonin (5-hydroxytryptamine). Ligand binding activates second messengers that inhibit the activity of adenylate cyclase and manage the release of serotonin, dopamine, and acetylcholine in the brain. The encoded protein may be involved in several neuropsychiatric disorders and therefore is often a target of antidepressant and other psychotherapeutic drugs.

Immunogen: E.coli-derived human 5HT1B/HTR1B recombinant protein (Position: A35-S390).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Bartsch, T., Knight, Y. E., Goadsby, P. J. Activation of 5-HT(1B/1D) receptor in the periaqueductal gray inhibits nociception. Ann. Neurol. 56: 371-381, 2004. 2. Demchyshyn, L., Sunahara, R. K., Miller, K., Teitler, M., Hoffman, B. J., Kennedy, J. L., Seeman, P., Van Tol, H. H. M., Niznik, H. B. A human serotonin 1D receptor variant (5HT1D-beta) encoded by an intronless gene on chromosome 6. Proc. Nat. Acad. Sci. 89: 5522-5526, 1992. 3. Garcia-Nafria, J., Nehme, R., Edwards, P. C., Tate, C. G. Cryo-EM structure of the serotonin 5-HT(1B) receptor coupled to heterotrimeric G(o). Nature 558: 620-623, 2018.

Uniprot ID: P28222

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 50 kDa

Calculated Molecular Weight: 18080 MW

Gene ID: 3351

Protein Name: 5-hydroxytryptamine receptor 1B

Gene Full Name: 5-hydroxytryptamine receptor 1B

Synonyms: 5 HT 1B; 5 HT 1D beta; 5 HT1B; 5 HT1DB; HTR1B; HTR1D2; HTR1DB; S12; Serotonin 1D beta receptor; Serotonin receptor 1B