ANGPTL8 ELISA kit (Human)

SKU: SEW803Hu

Size: 96Tests

# of Times Cited in literature: 15

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: ANGPTL8

Target Full Name: Angiopoietin Like Protein 8

Alternative Names: RIFL; TD26; C19orf80; Lipasin; Betatrophin; Hepatocellular Carcinoma-Associated Protein TD26

Target Species: Human

Uniprot: Q6UXH0

Gene ID: 55908

Featured Series: SE kit

Featured Series Function: Detects protein (regular version)

Specificity: Reactive with Human ANGPTL8 / Angiopoietin Like Protein 8

Method: Colormetric

Detection principle: Double-antibody Sandwich

Detection range: 0.156-10ng/mL

Sensitivity: 0.060ng/mL

Assay Time: 3h

Sample Size: 100uL

Recommended/Predicted Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiopoietin Like Protein 8 (ANGPTL8) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiopoietin Like Protein 8 (ANGPTL8) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Angiopoietin Like Protein 8 (ANGPTL8). No significant cross-reactivity or interference between Angiopoietin Like Protein 8 (ANGPTL8) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiopoietin Like Protein 8 (ANGPTL8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Angiopoietin Like Protein 8 (ANGPTL8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Angiopoietin Like Protein 8 (ANGPTL8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Angiopoietin Like Protein 8 (ANGPTL8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: Developmental science;

References Citing This Product: Increased Maternal and Cord Blood Betatrophin in Gestational Diabetes

Serum betatrophin level increased in subjects with metabolic syndrome: a case-control study

Serum betatrophin level increased in subjects with nonalcoholic fatty liver disease

Relationship between serum betatrophin levels and the first-phase of glucose-stimulated insulin secretion

Aumento del nivel de betatrofina en suero en sujetos con síndrome metabólico: estudio de casos y controles

Levels of betatrophin decrease during pregnancy despite increased insulin resistance, beta-cellfunction and triglyceride levels.

Decreased circulating levels of betatrophin in Chinese women with polycystic ovary syndrome

Serum concentrations of betatrophin and its association with indirect indices of insulin resistance and beta cell function in women with polycystic ovary …

Angiopoietin-like protein 8: an attractive biomarker for the evaluation of subjects with insulin resistance and related disorders

ANGPTL8 (Betatrophin) Role in Diabetes and Metabolic Diseases

The Correlation Between Circulating Betatrophin and Insulin Resistance in General Population: A Meta-Analysis

ANGPTL8: an important regulator in metabolic disorders

The Impact of Psoriasis and Metabolic Syndrome on the Systemic Inflammation and Oxidative Damage to Nucleic Acids

Genetic and Metabolic Determinants of Plasma Levels of ANGPTL8

Association of betatrophin amounts with 25-(OH) D levels in patients with gestational diabetes mellitus