AF/LE Purified Anti-Human IL-2 Antibody[MQ1-17H12]

SKU: GBS-F12000

Size:1mg

Storage:Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Shipping:Ice bag

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Flow Cytometry Antibodies

Abbreviation:IL-2

Target Synonym:IL2;IL-2;TCGF;lymphokine;Aldesleukin;IL-2lymphokine;interleukin-2;Proleukin;interleukin 2;involved in regulation of T-cell clonal expansion;T cell growth factor;T-cell growth factor;IL 2;IL2;Interleukin 2;interleukin2;Involved in regulation of T cell clonal expansion;Lymphokine;POIL2;T Cell Growth Factor;TCGF

Research Areas:Immunology

Conjugation:None (AF/LE)

Host:Rat

Species reactivity:Human

Application:FCM

Usage:

Isotype:Rat IgG2a, κ

Clonality:Monoclonal

Clone NO.:MQ1-17H12

UNIProt ID:P60568

Background:Interleukin-2 (IL-2) is a O-glycosylated, four alpha -helix bundle cytokine that has potent stimulatory activity for antigen-activated T cells. It is expressed by CD4+ and CD8+ T cells, gamma δ T cells, B cells, dendritic cells, and eosinophils. Mature human IL-2 shares 56% and 66% aa sequence identity with Mouse and Rat IL-2, respectively. Human and Mouse IL-2 exhibit cross-species activity. The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes. The 55 kDa IL-2 R alpha is specific for IL-2 and binds with low affinity. The 75 kDa IL-2 R beta, which is also a component of the IL-15 receptor, binds IL-2 with intermediate affinity. The 64 kDa common gamma chain gamma c/IL-2 R gamma, which is shared with the receptors for IL-4, -7, -9, -15, and -21, does not independently interact with IL-2. Upon ligand binding, signal transduction is performed by both IL-2 R beta and gamma c. IL-2 is best known for its autocrine and paracrine activity on T cells. It drives resting T cells to prolifeRate and induces IL-2 and IL-2 R alpha synthesis. It contributes to T cell homeostasis by promoting the Fas-induced death of naïve CD4+ T cells but not activated CD4+ memory lymphocytes. IL-2 plays a central role in the expansion and maintenance of regulatory T cells, although it inhibits the development of Th17 polarized cells. Thus, IL-2 may be a key cytokine in the natural suppression of autoimmunity.

Concentration:≥ 1 mg/mL

Control catalog number:

Immunogen:Recombinant Human IL-2 protein

Buffer:Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method.

Purification method:>98%, Protein A/G purified

Dilution:FCM 2 μg/mL(0.5×10⁶-1×10⁶ cells)

GeneID:3558